Year: 2018 | Month: April | Volume 8 | Issue 2
Genetic Diversity and Phylogenetic Relationship Analysis of two Rabbit Breeds by Microsatellite Markers
Abstract:
Soviet Chinchilla (SC) and Californian White (CW) breeds of rabbits maintained in the Rabbit research Centre, college of Veterinary Science, Rajendranagar, Hyderabad were utilized for the present study. Rabbit specific microsatellite primers utilized were successfully amplified in both the breeds by PCR and these primers are highly polymorphic and informative with number of alleles ranged from 4 to 11 in SC and 6 to 10 in CW. A total of 199 alleles (102 in SC and 97 in CW) were observed across the amplified loci. The overall mean of observed, expected and unbiased expected heterozygosity values were 0.681, 0.842 and 0.872 in SC and 0.665, 0.849 and 0.880 in CW, respectively. The mean inbreeding coefficient was 0.194 in SC and 0.221 in CW. Only 1 locus showed negative inbreeding coefficient in both the breeds. The mean PIC was 0.822 and 0.831 in SC and CW breeds respectively. The overall mean FIS, FIT and FST values were 0.208, 0.238 and 0.040 respectively. At two loci SAT07 and SAT16 moderate and high degree of differentiation was observed between the two breeds and all the remaining 10 loci have the FST values less than 0.05 suggesting that differentiation did not exist between the two breeds at these loci. The sufficiently high mean values of observed number of alleles, observed heterozygosity and PIC for various microsatellites in the present study supported their suitability for genetic diversity and phylogenetic relationship studies.
© This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
Print This Article Email This Article to Your Friend
